| Ryan Eggold | Dr. Max Goodwin |
| Janet Montgomery | Dr. Lauren Bloom |
| Freema Agyeman | Dr. Helen Sharpe |
| Jocko Sims | Dr. Floyd Reynolds |
| Tyler Labine | Dr. Iggy Frome |
| Michelle Forbes | Dr. Veronica Fuentes |
| Smith Alfieri | Lazerfox |
| Peter Hans Benson | Gary Burgess |
| Jaspal Binning | Garrett Gardam |
| Megan Byrne | Gladys |
| Christine Chang | Dr. Agnes Kao |
| Sandra Mae Frank | Dr. Wilder |
| Alejandro Hernandez | Casey Acosta |
| Amber Hodgkiss | Maureen Gardam |
| Matthew Jeffers | Dr. Mark Walsh |
| Shiva Kalaiselvan | Leyla |
| Keren Lugo | Dr. Diana Flores |
| Conner Marx | Ben Meyer |
| America Olivo | Inez Vea |
| Michael Siberry | Roderick Cantleigh |
| Anna Suzuki | Sandra Fall |
| Leslie Uggams | Mama Reynolds |
| Debra Monk | Karen Brantley |
| Morgan Assante | Patty the Patient Care Tech |
| Becca Ballenger | Paramedic Goodrich |
| Dimitri Carter | Orderly James |
| Lawrence Oliver Cherry | Deacon Earnest |
| Brenda Crawley | Maggie from Maternity |
| Mahalet Dejene | Anesthesiologist Nyala Abebe |
| Donna Glaesener | Janitor Astrid |
| Ben Hollandsworth | Adam B |
| Zac Jaffee | Larry from Clerical |
| Olivia Khoshatefeh | Dr. Yasmin Turan |
| Joseph Latimore | Maintenance Worker Nando |
| Allegra Leguizamo | Emma |
| Tim Liu | Rodney from IT |
| Gemma McIlhenny | Maintenance Worker Janice |
| Eduardo Navas | Maintenance Worker Rafael |
| Atibon Nazaire | Maintenance Worker Carl |
| Amanda Kristin Nichols | Elaina the OR Tech |
| Tyrone L. Robinson | Custodian Jerry Watkins |
| Reece Santos | Cafeteria Tony |
| Isaiah Seward | Paramedic Durango |
| Mahadeo Shivraj | Jin the Janitor |
| Peter Stray | Dr. Phil Gabriel |
| Darren Valinotti | Ollie Gardam |
| Veronica Wathome | Maintenance Marta |
| Jennifer Betit Yen | OR Nurse Aimee Kamoe |
| Erick Zamora | Matias Reyes |