| Mark Harmon | Leroy Jethro Gibbs |
| Michael Weatherly | Anthony DiNozzo |
| Cote de Pablo | Ziva David |
| Pauley Perrette | Abby Sciuto |
| Sean Murray | Timothy McGee |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Michael O'Neill | Former NCIS Special Agent Riley McCallister |
| Catherine Dent | Whitney Sharp |
| T.J. Ramini | Mossad Officer Malachi Ben-Gidon |
| Sarai Givaty | Mossad Officer Liat Tuvia |
| Oren Dayan | Karif Yasin |
| Eric Ramey | Dr. Nolan Cooper |
| Michael Nouri | Mossad Director Eli David |
| Stan Ivar | Former NCIS Special Agent Ben Robinson |
| Tristen MacDonald | Nurse Davies |
| Carolyn Ratteray | Nurse |
| Arnold Vosloo | Mossad Officer Amit Hadar |
| Andrew Kirsanov | The Russian |
| Abraham Justice | Abasi Al-Masri |
| Cody Kennedy | Natasha |
| Lauren Holly | Jenny Shepard |
| Patrick Weil | Special Agent |