| Mark Harmon | Leroy Jethro Gibbs |
| Michael Weatherly | Anthony DiNozzo |
| Pauley Perrette | Abby Sciuto |
| Sean Murray | Timothy McGee |
| Brian Dietzen | Jimmy Palmer |
| Emily Wickersham | Ellie Bishop |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Jon Cryer | Dr. Cyril Taft |
| Scottie Thompson | Jeanne Benoit |
| Joel Gretsch | NCIS Special Agent Stan Burley |
| David Chisum | Dr. David Woods |
| Annie Tedesco | Navy Doctor Lieutenant Liz Cortland |
| Gabriel Hogan | Navy Seal Commander Pete Grady |
| Katie Roberts | Navy Surgical Tech Ensign Joni Ryan |
| Omono Okojie | Bahar Tambai |
| Noel Arthur | Ramses |
| Sarah Jane MacKay | Rose |
| Akuyoe Graham | Zayah |
| Jerald Garner | Insurgent |
| Knute Horwitz | Dr. Felix Pitts |
| Kortney Manns | Insurgent |