| Mark Harmon | Leroy Jethro Gibbs |
| Michael Weatherly | Anthony DiNozzo |
| Pauley Perrette | Abby Sciuto |
| Sean Murray | Timothy McGee |
| Brian Dietzen | Jimmy Palmer |
| Emily Wickersham | Ellie Bishop |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Kelli Williams | NCIS Special Agent Maureen Cabot |
| Gregory Harrison | Captain Roland Ebbakey |
| Margo Harshman | Delilah Fielding |
| Brad Benedict | Lieutenant Nick Rossmore |
| Kelly Frye | Lieutenant Kara Gifford |
| Ryan Kennedy | Former Navy Petty Officer First Class Peter Woodruff |
| Andrew Hawkes | Metro P.D. Detective Dan Campbell |
| Alisha Boe | Farrah Meyers |
| Dante Swain | Petty Officer Second Class Adam Meyers |
| Makabe Ganey | Jackson Edward |
| Melissa Greenspan | Mother |
| Jamie Bamber | NSA Attorney Jake Malloy |
| Phillip E. Walker | Coffee Shop Patron |