| Ben McKenzie | James Gordon |
| Donal Logue | Harvey Bullock |
| David Mazouz | Bruce Wayne |
| Morena Baccarin | Leslie Thompkins |
| Sean Pertwee | Alfred Pennyworth |
| Robin Lord Taylor | Oswald Cobblepot /
Penguin |
| Erin Richards | Barbara Kean |
| Camren Bicondova | Selina Kyle |
| Cory Michael Smith | Edward Nygma /
The Riddler |
| Jessica Lucas | Tabitha Galavan |
| Chris Chalk | Lucius Fox |
| Drew Powell | Solomon Grundy |
| Crystal Reed | Sofia Falcone |
| Alexander Siddig | Ra's al Ghul |
| Cameron Monaghan | Jerome Valeska /
Jeremiah Valeska |
| Benedict Samuel | Jervis Tetch |
| Nathan Darrow | Victor Fries /
Mr. Freeze |
| Michelle Veintimilla | Bridgit Pike /
Firefly |
| Shiva Kalaiselvan | Lelia |
| David W. Thompson | Jonathan Crane /
Scarecrow |
| Peter McRobbie | Mayor Holden Pritchard |
| Neimah Djourabchi | Tall Man |
| Alison Fraser | Gertrude Haverstock |
| Harry Sutton Jr. | Commissioner |
| Brenny Rabine | Gloria Bainbridge |
| Brian Lee Huynh | Zhang |
| Steve Lord | SWAT Leader |
| Adam Sinclair | Johnny Spectre |
| Jane Kahler | Chemical Engineer |
| Erik McKay | Co-Pilot |
| Joseph Dellger | Pilot |
| Jo D. Jonz | Wyatt |
| Karilyn Ashley Surratt | Dancer #2 |
| Lisa Jantzie | Dancer #1 |
| Reg Clinton Brown | Escaped Arkham Inmate
(uncredited) |
| Christopher Convery | Martin
(uncredited) |
| Regina Hardy | Narrowrite
(uncredited) |
| Robert Leckington | Drummer
(uncredited) |
| Judy Malka | Gotham Citizen
(uncredited) |
| Kenneth Kyle Martinez | Gothamite
(uncredited) |
| Kevin D. McGee | GCPD Officer
(uncredited) |
| Fermin Padilla III | Loon
(uncredited) |
| Billy Peck | Strike Force
(uncredited) |
| Stuart Schnitzer | Core Desk Sergeant
(uncredited) |
| Kelly P. Williams | Gotham City Police
(uncredited) |