| Ben McKenzie | James Gordon |
| Donal Logue | Harvey Bullock |
| David Mazouz | Bruce Wayne |
| Morena Baccarin | Leslie Thompkins
(credit only) |
| Sean Pertwee | Alfred Pennyworth |
| Robin Lord Taylor | Oswald Cobblepot /
Penguin |
| Erin Richards | Barbara Kean |
| Camren Bicondova | Selina Kyle |
| Cory Michael Smith | Edward Nygma |
| James Frain | Theo Galavan
(credit only) |
| Jessica Lucas | Tabitha Galavan
(credit only) |
| Chris Chalk | Lucius Fox |
| Drew Powell | Butch Gilzean |
| Nicholas D'Agosto | Harvey Dent
(credit only) |
| Michael Chiklis | Nathaniel Barnes
(credit only) |
| Jada Pinkett Smith | Fish Mooney |
| BD Wong | Hugo Strange |
| Tonya Pinkins | Ethel Peabody |
| Nathan Darrow | Mr. Freeze |
| Michelle Veintimilla | Firefly |
| Kit Flanagan | The White Haired Woman |
| Radu Spinghel | Scale Skin Man |
| Gerard Cordero | Strike Force Lieutenant |
| Marcia Berry | Old Lady Hobo |
| Brette Taylor | Martha Wayne |
| Scott Brody | Caged One Eyed Monster
(uncredited) |
| Anthony Cumberbatch Jr. | Grape Monster
(uncredited) |
| Stanley Krajewski | Arkham Prison Guard
(uncredited) |
| Mike Malvagno | Strike Force
(uncredited) |
| Robert Lil Bob McCall | Little Person Monster
(uncredited) |
| Grayson McCouch | Dr. Thomas Wayne
(archive footage) (uncredited) |
| Frank Modica | GCPD CORE Detective
(uncredited) |
| Kelly P. Williams | Gotham City Police
(uncredited) |