| Ben McKenzie | James Gordon |
| Donal Logue | Harvey Bullock |
| David Mazouz | Bruce Wayne |
| Morena Baccarin | Leslie Thompkins |
| Zabryna Guevara | Sarah Essen |
| Sean Pertwee | Alfred Pennyworth |
| Robin Lord Taylor | Oswald Cobblepot /
Penguin |
| Erin Richards | Barbara Kean |
| Camren Bicondova | Selina Kyle |
| Cory Michael Smith | Edward Nygma |
| James Frain | Theo Galavan |
| Jessica Lucas | Tabitha Galavan |
| Cameron Monaghan | Jerome Valeska |
| Peter Scolari | Commissioner Loeb |
| Anthony Carrigan | Victor Zsasz |
| Dustin Ybarra | Robert Greenwood |
| Todd Stashwick | Richard Sionis |
| Will Brill | Dobkin |
| David Fierro | Zaardon |
| Otto Sanchez | Odgen Barker |
| James Andrew O'Connor | Tommy Bones |
| Stink Fisher | Aaron Helzinger |
| Philip Goodwin | Compere |
| Drew Powell | Butch Gilzean |
| Michael Barra | Officer Franks |
| Giuseppe Ardizzone | Arkham Asylum Guard
(uncredited) |
| Reg Clinton Brown | Arkham Inmate
(uncredited) |
| Stanley Krajewski | Arkham Prison Guard
(uncredited) |
| Kelly P. Williams | Gotham City Police
(uncredited) |
| Rocco Wu | Prep School Boy
(uncredited) |