| Dianna Agron | Quinn Fabray |
| Chris Colfer | Kurt Hummel |
| Jessalyn Gilsig | Terri Schuester |
| Jane Lynch | Sue Sylvester |
| Jayma Mays | Emma Pillsbury |
| Kevin McHale | Artie Abrams |
| Lea Michele | Rachel Berry |
| Cory Monteith | Finn Hudson |
| Matthew Morrison | Will Schuester |
| Amber Riley | Mercedes Jones |
| Mark Salling | Noah 'Puck' Puckerman |
| Jenna Ushkowitz | Tina Cohen-Chang |
| Josh Groban | Self |
| Jonathan Groff | Jesse St. James |
| Idina Menzel | Shelby Corcoran |
| Iqbal Theba | Principal Figgins |
| Charlotte Ross | Judy Fabray |
| Naya Rivera | Santana Lopez |
| Bill A. Jones | Rod Remington |
| Heather Morris | Brittany S. Pierce |
| Harry Shum Jr. | Mike Chang |
| Dijon Talton | Matt Rutherford |
| Olivia Newton-John | Self |
| Carole Gutierrez | Nurse #1 |
| Jennifer Say Gan | Nurse #2 |
| Capt'n Bob | Parent |
| Todd Bouldin | Parent |
| Tristan James Bower | Vocal Adrenaline Guitarist |
| Ian Brennan | Narrator |
| Taisha Monique Clark | Giselle |
| Kara DeFrias | New Directions Fan |
| Nick Drago | Vocal Adrenaline Dancer |
| Korey Dunaway | Dancer |
| Brad Ellis | Brad the Piano Player |
| Lauren Gottlieb | Vocal Adrenaline Dancer |
| Gayle Kate | Parent of Student |
| Quinn Lipton | Vocal Adrenaline Dancer |
| Chase McKendry | New Directions Fan |
| Mike Munich | Dancer |
| Vivian Nixon | Andrea Cohen |
| Doug Penikas | Vocal Adrenaline Dancer |
| Jameson Perry | Dancer |
| Courtney Platt | Vocal Adrenaline Dancer |
| Stefan Poole | Vocal Adrenaline Lead Guitarist |
| Shelby Rabara | Shoshandra |
| Sonya Rome | Vocal Adrenaline performer |
| Chas Scherer | Medical Technician |
| J.A. Steel | Parent of Student |
| Sam Tan | Hockey Jock |