| Michael C. Hall | Dexter Morgan |
| Jennifer Carpenter | Debra Morgan |
| Desmond Harrington | Det. Joseph 'Joey' Quinn |
| C.S. Lee | Vince Masuka |
| David Zayas | Lt. Angel Batista |
| Aimee Garcia | Jamie Batista |
| Geoff Pierson | Deputy Chief Tom Matthews |
| James Remar | Harry Morgan
(credit only) |
| Yvonne Strahovski | Hannah McKay |
| Sean Patrick Flanery | Jacob Elway |
| Amy Pietz | Dr. Cooper |
| Eric Ladin | Dr. Turner |
| Darri Ingolfsson | Oliver Saxon |
| Jadon Wells | Harrison Morgan |
| Marc Gomes | Dr. Willis |
| Suzanne Whang | Anchor Woman |
| Mercedes López Renard | Gate Attendant
(as Mercedes Renard) |
| Jernard Burks | Driver |
| Charles Carpenter | Reporter |
| Skye P. Marshall | Receptionist |
| Jerry Ying | Paramedic #1 |
| Brian Oblak | TSA Agent #1 |
| Alexandra Hoover | Female Uni |
| David Haley | Security Guard |
| Mary-Bonner Baker | Nurse Jansen |
| Rachelle Pettinato | Nurse
(as Rachelle Wells) |
| Sergio Garcia | Latino Man |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |
| Lori Morkunas Jones | Buenos Aires Street Walker
(uncredited) |
| Darren P. Leis | Nurse
(uncredited) |
| Mariya Ranieli | Nurse
(uncredited) |
| Annette Marie Westwood | Neonatal Nurse
(uncredited) |