| Michael C. Hall | Dexter Morgan |
| Julie Benz | Rita Morgan |
| Jennifer Carpenter | Debra Morgan |
| Desmond Harrington | Det. Joseph 'Joey' Quinn |
| C.S. Lee | Vince Masuka |
| Luna Lauren Velez | Lt. Maria LaGuerta
(as Lauren Velez) |
| David Zayas | Sgt. Angel Batista |
| James Remar | Harry Morgan |
| John Lithgow | Arthur Mitchell |
| David Ramsey | Anton Briggs |
| Courtney Ford | Christine Hill |
| Suzanne Cryer | Tarla Grant |
| J.C. MacKenzie | Andy Brightman |
| Keith Carradine | Frank Lundy |
| Christina Robinson | Astor Bennett |
| Preston Bailey | Cody Bennett |
| Rick Peters | Elliot |
| Matthew Fahey | Jesse Brightman |
| Marisa Petroro | Kate |
| Alicia Lagano | Nikki Wald |
| Ricardo Chacon | Deputy Russell
(as Ricardo J. Chacon) |
| Matthew R. Anderson | Neighborhood Watch Member
(uncredited) |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |