| Michael C. Hall | Dexter Morgan |
| Julie Benz | Rita Bennett |
| Jennifer Carpenter | Debra Morgan |
| Erik King | Sgt. James Doakes |
| C.S. Lee | Vince Masuka |
| Luna Lauren Velez | Det. Maria LaGuerta
(as Lauren Velez) |
| David Zayas | Det. Angel Batista |
| James Remar | Harry Morgan |
| Geoff Pierson | Capt. Tom Matthews |
| JoBeth Williams | Gail Brandon |
| Judith Scott | Lt. Esme Pascal |
| Dominic Janes | Young Dexter |
| John Marshall Jones | Curtis Barnes |
| Bertila Damas | Soto's Widow |
| Albie Selznick | Psychiatrist |
| Jaime Murray | Lila Tournay |
| Keith Carradine | Special Agent Frank Lundy |
| Christina Robinson | Astor Bennett |
| Preston Bailey | Cody Bennett |
| Dave Baez | Gabriel |
| Kathrin Middleton | Doris Morgan
(as Kathrin Lautner Middleton) |
| Mario Marcelino | Dr. Pena |
| Charles Perez | Male Commentator |
| Calvin Hughes | Male News Show Host |
| Jimmy Star | FBI Agent |
| Kiko Ellsworth | Bertrand
(uncredited) |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |