| Michael C. Hall | Dexter Morgan |
| Julie Benz | Rita Bennett |
| Jennifer Carpenter | Debra Morgan |
| Erik King | Sgt. James Doakes |
| Luna Lauren Velez | Lt. Maria LaGuerta
(as Lauren Velez) |
| David Zayas | Det. Angel Batista |
| James Remar | Harry Morgan |
| C.S. Lee | Vince Masuka |
| Valerie Dillman | Valerie Castillo |
| Vernee Watson | Doakes' Mother
(as Vernée Watson Johnson) |
| Andi Chapman | Social Worker |
| José Zúñiga | Jorge Castillo
(archive footage) (as Jose Zuniga) |
| Christina Robinson | Astor Bennett |
| Daniel Goldman | Cody Bennett |
| Devon Graye | Teenage Dexter |
| Hunter King | Teenage Debra
(as Haley King) |
| Cesar Flores | Oscar |
| Damian T. Raven | Forbes |
| Minerva García | Mariel
(as Minerva Garcia) |
| Kelli Kirkland | Jess
(as Kelli Kirkland Powers) |
| Lyn Alicia Henderson | Roni |
| Demetrius Grosse | Alex Timmons |
| Benton Jennings | Gene Marshall |
| Julie Dolan | Cindy Landon |
| Gabriel Salvador | Roberto |
| Denver Dowridge | Police Cadet |
| Gabriel Cordell | Sketch Artist |
| Wing Armstrong | Medical Examiner Munoz
(uncredited) |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |
| Chris Muto | Police Officer
(uncredited) |
| Mark Pellegrino | Paul Bennett
(voice) (uncredited) |
| Frank Tedesco | Miami Dade Police Officer
(uncredited) |