| William Petersen | CSI Dr. Gil Grissom |
| Marg Helgenberger | C.S.I. Night Shift Assistant Supervisor Catherine Willows |
| Gary Dourdan | Warrick Brown |
| George Eads | Nick Stokes |
| Jorja Fox | Sara Sidle |
| Eric Szmanda | Greg Sanders |
| Robert David Hall | Al Robbins |
| Paul Guilfoyle | Captain Jim Brass |
| Paul Dooley | Buddy Ween |
| Heather Kafka | Mindy DuPont |
| José Zúñiga | Detective Chris Cavaliere |
| Judith Hoag | Merill Maguire |
| Rocco Sisto | Riley 'Boom Boom' Renaldo - Mindy's Landlord |
| Joe Chrest | Al Maguire |
| Keram Malicki-Sánchez | Jamal |
| David Berman | David Phillips |
| Debra Wilson | Divine |
| Matthew Walker | The Mime |
| Nicholas Lea | Chris Bezich |
| James Russo | Sinclair |
| Kevin C. Carr | Donny 'Doodles' Zanko |
| Erin Foster | Crackhead Girl |
| Ross Partridge | Ed Burnell |