| David Duchovny | Fox Mulder |
| Gillian Anderson | Dana Scully |
| Fiona Vroom | Barbara Beaumont /
Sitcom Barbara |
| Carlena Britch | Juliet 'La Avispa' Bocanegra |
| Jere Burns | Dr. Randolph Luvenis |
| Viv Leacock | Price |
| Micaela Aguilera | Olivia Bocanegra |
| Samuel Patrick Chu | Warren |
| Fabiola Colmenero | Josephine Bocanegra |
| Genevieve Buechner | Kayla |
| Cameron McDonald | Father Hardy |
| Albert Nicholas | Agent Bill Bludworth |
| Angus Stuart | Cathedral Priest |
| Aidan Kahn | Agent Colquitt |
| Jay Hindle | Sitcom Joe |
| Garrett Black | Technician |
| Guy Fauchon | Dr. Dave Rednon |
| Neil Corbett | Intern |
| Nick Heffelfinger | Calvin |
| Joel McHale | Tad O'Malley
(voice) (uncredited) |
| Nailya Red | Cult Member
(uncredited) |