| Tina Fey | Liz Lemon |
| Tracy Morgan | Tracy Jordan |
| Jane Krakowski | Jenna Maroney |
| Jack McBrayer | Kenneth Parcell |
| Scott Adsit | Pete Hornberger |
| Judah Friedlander | Frank Rossitano |
| Alec Baldwin | Jack Donaghy |
| Katrina Bowden | Cerie |
| Keith Powell | Toofer |
| Kevin Dotcom Brown | Dot Com |
| Grizz Chapman | Grizz |
| John Lutz | J.D. Lutz |
| Chris Parnell | Dr. Leo Spaceman |
| Amy Poehler | Young Liz |
| Kristen Schaal | Hazel Wassername |
| Brian Williams | David Brinkley (West Coast only) |
| Sue Galloway | Sue |
| Cheyenne Jackson | Danny Baker |
| Fred Armisen | Frank 2.0 |
| Jimmy Fallon | Young Jack |
| Will Forte | Paul |
| Jon Hamm | Abner |
| Kim Kardashian West | Kim Kardashian (West Coast only) |
| Paul McCartney | Paul McCartney (East Coast only) |
| Patrick Cotnoir | Self - Audience Member |
| Donald Glover | Young Tracy Jordan |